This volume presents information about protocols for micropropagation of more than 40 species of medicinal plants. The contents combine knowledge about the scientific principles of micropropagation with state of the art updates in tissue culture techniques presented by plant scientists.
The readers will learn about techniques required to grow plants in challenging conditions that aim to reduce the impacts of injudicious harvesting, deforestation, climate change, pollution, urbanization and other factors that limit the ability to meet current demand. General topics such as biotization and pharmaceutical investigation are also included to guide readers about the significance of these plants in research and development for new medicines.
The book provides protocols for micropropagation of important medicinal plants like Rauvolfia serpentina, Catharanthus roseus, Withania somnifera, Tylophora indica, Bacopa monnieri, Aloe vera, Phyllanthus amarus, Allium sativum, Moringa oleifera, Operculina turpethum, Glycyrrhiza glabra, Pterocarpus marsupium, Vetiver grass, Ruta graveolens, Tinospora cordifolia, Kaempferia, Hedychium, Decalepis hamiltonii, Saraca asoca, Wrightia tinctoria, Wrightia arborea, Artemisia absinthium, Aegle marmelos, Atropa acuminata, Atropa belladonna, Alpinia species, Hedychium species, and Cissus species.
This book is a handy reference for medicinal chemists, horticulturists and pharmacists who want to learn about the growth and conservation of important medicinal herbs and plants.
The readers will learn about techniques required to grow plants in challenging conditions that aim to reduce the impacts of injudicious harvesting, deforestation, climate change, pollution, urbanization and other factors that limit the ability to meet current demand. General topics such as biotization and pharmaceutical investigation are also included to guide readers about the significance of these plants in research and development for new medicines.
The book provides protocols for micropropagation of important medicinal plants like Rauvolfia serpentina, Catharanthus roseus, Withania somnifera, Tylophora indica, Bacopa monnieri, Aloe vera, Phyllanthus amarus, Allium sativum, Moringa oleifera, Operculina turpethum, Glycyrrhiza glabra, Pterocarpus marsupium, Vetiver grass, Ruta graveolens, Tinospora cordifolia, Kaempferia, Hedychium, Decalepis hamiltonii, Saraca asoca, Wrightia tinctoria, Wrightia arborea, Artemisia absinthium, Aegle marmelos, Atropa acuminata, Atropa belladonna, Alpinia species, Hedychium species, and Cissus species.
This book is a handy reference for medicinal chemists, horticulturists and pharmacists who want to learn about the growth and conservation of important medicinal herbs and plants.
Readership
Medicinal chemists, horticulturists and pharmacists.Table of Contents
Chapter 1 Biotization of Medicinal Plant Cultures by Endophytes: A Promising Approach to Enrich Therapeutics- Srinivasan T. Rajya Lakshmi Y.V. Shanmukha Anand P. Sunitha P. And Aruna Lakshmi K.
- Introduction
- Need for Micropropagation of Medicinal Plants
- Micropropagation of Tissue and Organ Culture
- Mass Cell Cultures in Bioreactors
- Genetic Transformation for the Production of Metabolites
- Current Trade/Economical Aspects of Plant Tissue Culture (Ptc) Medicinal Plants in India And the World
- Future Needs for Propagation
- General Protocol for Micropropagation of Medicinal Plants
- Nutrition Media
- Surface Sterilization of the Explants
- Culture Conditions
- Plantlet Formation and Acclimatization
- Assessment of Regenerants
- Liquid Suspension Cultures for Metabolites
- Aberrant Features/Characteristics of Tissue Culture Plantlets
- What Are Endophytes?
- Diversity in the Endophyte Population
- Isolation of Endophytes
- Endophytes from Medicinal Plants
- In Vitro Biotization of Medicinal Plants
- Effect of Microbial Endophytes on the Growth and Secondary Metabolite Production of In
- Vitro Plants
- Host -Endophyte Associations on the Secondary Metabolites Production
- Conclusion and Future Prospects
- References
- Introduction
- Micropropagation
- Explant Sterilization
- Nutrient Medium and Response
- Rooting Medium
- Acclimatization
- Genetic Fidelity
- Conclusion
- References
- (L.) Dunal - An Important Medicinal Plant
- V. Rajasreelatha, M. Thippeswamy, M. Siva Kumar, V. Naga Padmavathi and T.
- Pullaiah
- Introduction
- Chemical Compounds in Withania Somnifera
- Significance of in Vitro Regeneration of Withania Somnifera
- In Vitro Studies of W. Somnifera
- Chemicals and Sterilization
- Seed Germination
- Callus Culture Studies in W. Somnifera
- In Vitro Shoot Multiplication
- Conclusion
- Abbreviations
- References
- Rodrigo Oliveira Almeida
- Introduction
- Aloe Vera Applications
- Aloe Vera Tissue Culture
- Explant
- Disinfection
- Culture Medium
- Growth Chamber Parameters
- Acclimatization
- Concluding Remarks
- References
- Schum. & Thonn.
- S. Anitha
- Introduction
- Micropropagation Protocols
- Explant Sterilization
- Nutrient Medium and Response
- Rooting
- Acclimatization
- Conclusion
- References
- Koenig (Zingiberaceae)
- S. Vani, Sinjumol Thomas and Bince Mani
- Introduction
- Micropropagation of Hedychium Species
- Hedychium Bousigonianum Pierre Ex Gagnep
- Somatic Embryogenesis
- Hedychium Coccineum Buch.-Ham. Ex Sm.
- Direct Organogenesis
- Callogenesis
- Somatic Embryogenesis
- Hedychium Coronarium J. Koenig
- Multiple Shoot Regeneration Through Direct Organogenesis
- Seed Culture and Direct Organogenesis
- Embryo Culture
- Microrhizome Induction and Adventitious Shoot Production
- Somatic Embryogenesis
- In Vitro Polyploidization
- Impact of Additives on Plant Regeneration
- Hedychium Ellipticum Buch.-Ham. Ex Sm.
- Direct Organogenesis from Leafy Shoot Bases
- Hedychium Flavum Roxb.
- Shoot Culture
- Hedychium Forrestii Diels
- Embryo Culture
- Hedychium Longicornutum Griff. Ex Baker
- Direct Organogenesis and Callogenesis
- Hedychium Muluense R.M.Sm.
- Somatic Embryogenesis
- In Vitro Polyploidization
- Hedychium Roxburghii Blume
- Rhizome-Meristem Culture
- Hedychium Spicatum Sm.
- Multiple Shoot Induction Through Direct Organogenesis
- Somatic Embryogenesis
- Hedychium Stenopetalum G.Lodd.
- Shoot Tip Culture
- Conclusion
- References
- Rasha N. Arafa and Sayed A. A. Elsayh
- Introduction
- Physical Characteristics
- J. Curcas Therapeutic Properties
- Propagation of Jatropha by Tissue Culture
- Materials and Methods
- Stage (1): Germination Seeds in Vivo
- Stage (2): Tissue Culture Stage
3. Sterilization of Explants
4. Culture Media
5. Callus Induction
6. Differentiation Stage
- Stage (3). Multiplication Stage
- Stage (4). Rooting Stage
- Stage (5). Acclimatization
- Results and Discussion
- Surface Sterilization of Explants
- Callus Induction
- Differentiation Stage
- Multiplication Stage
- Rooting Stage
- Acclimatization
- Conclusion
- References
- And Pharmacological Properties
- Sarita Kumari Rajani, Rishi Kesh Meena, Preeti Mishra and Vidya Patni
- Introduction
- Applications of Moringa Oleifera
- Geographical Distribution and Botanical Description
- Biochemical Profile
- Micro-Propagation in Moringa Oleifera Lam.
- Micro-Propagation Through Nodal Explants
- Micro-Propagation Through Stem Explant
- Micro-Propagation Through Leaf Explants
- Problems Associated With Micro-Propagation of the M. Oleifera
- Biological Activities of Moringa Oleifera
- Antidiabetic Properties
- Anticancerous Properties
- Antibacterial Activity
- Antiviral Activities
- Neuroprotective Effect
- Antioxidant Activity
- Hepato-Protective Activity
- Anti-Atherosclerotic Activities
- Future Scope
- Conclusion
- References
- L.V. Aswanilal, S.R. Saranya Krishnan and E. A. Siril
- Introduction
- Medicinal Importance of O. Umbellata
- Need for Biotechnological Interventions and in Vitro
- Propagation
- In Vitro Production of Anthraquinones (Aq) from O. Umbellata
- Conclusion
- References
- Lavr Kryukov, Andrey Vorotyntsev and Aleksandr Shirokov
- Introduction
- Materials and Methods
- Plant Material
- Dactylorhiza Baltica (Klinge) Orlova
- Dactylorhiza Incarnata (L.) Soó
- Dactylorhiza Fuchsii (Druce) Soo
- Dactylorhiza Maculata (L.) Soo.
- Dactylorhiza Traunsteineri (Saut.) Soo
Author
- T. P ullaiah
