This reference is a comprehensive review of genome editing in bacteria. The multi-part book meticulously consolidates research findings and insights on the applications of bacteria across several industries, including food processing and pharmaceutical development. The book covers four overarching themes for readers: a historical perspective of genome editing, genome editing in probiotics, applications of genome editing in agricultural microbiology and genetic engineering in environmental microbiology. The editors have also compiled chapters that provide an in-depth analysis of gene regulation and metabolic engineering through genome editing tools for specific bacteria.
Key topics in part 1:
- An Overview of advances in CRISPR-CAS research
- Applications of CRISPR/CAS9-based genome editing for industrial microorganisms
- Gut microbiome modulation to address gut dysbiosis
- Bifidobacterium genome editing for probiotic development and metabolic engineering.
- Insights into the use of lactic acid bacteria as starter cultures in the food
- Genome editing of vegetable-derived L. Plantarum
- Genome editing in Bacillus Licheniformis
Readership
Scholars and professionals interested in bacterial genomics.Table of Contents
Chapter 1 Recent Advances in Crispr-Cas Genome Engineering: An Overview- Angelina Job Kolady, Aritra Mukherjee, Ranjith Kumavath, Sarvepalli Vijay
- Kumar and Pasupuleti Sreenivasa Rao
- Introduction
- Genome Editing
- Genome Editing Tools and Their Limitations
- Restriction Enzymes (Re)
- Zinc Finger Nucleases (Znfs)
- Transcription Activator Like Effector Nucleases
- Discovery and History
- Components of Crispr-Cas
- Cas9
- Sgrna and How to Design Sgrnas
- Pam Sequence
- Component Crispr
- Component Crispr (3C Crispr)
- Application of Crispr
- Generation of Knockout
- Making Specific Modifications
- Combat Antimicrobial Resistance
- Genome Engineering in Agriculture
- Genome Engineering in Industrial Biotechnology
- Future Perspectives of Crispr-Cas9
- Limitation of Crispr-Cas
- Conclusion
- Acknowledgments
- References
- Kiran Dindhoria, Vivek Manyapu, Ashif Ali, Aman Kumar and Rakshak Kumar
- Introduction
- Origin of Crispr/Cas9 System
- Crispr-Cas Systems Classification
- Crispr/Cas Based Genome Editing Methods
- Gene Knock-Out
- Gene Knock-In
- Transcriptional Regulation
- Multiplex Editing
- Signalling Pathways
- Applications of Crispr/Cas9 in Different Types of Industries
- Agricultural Industry
- Biofuel and Other Industrial Products
- Food Industry
- Biotherapeutics
- Barriers to Genome Editing by Crispr/Cas9
- Absence of Efficient Dna Repair Methods
- Low Specificity
- Lack of Suitable Delivery Methods
- Ethical Issues
- Future Outlook
- Acknowledgments
- References
- Atul R. Chavan, Maitreyee Pathak, Hemant J. Purohit and Anshuman A.
- Khardenavis
- Introduction
- Crispr and Gut Microbiome Management by Prebiotics, Probiotics,
- And Synbiotics
- Conventional Omics Tools for Improved Prebiotics Production
- Conventional Omics Tools for Improved Probiotics Production
- Crispr Based Genetic Manipulation in Prebiotics and Probiotics Production
- Microbiome Editing Using Crispr
- Synthetic Biology Approaches to Microbiome Therapies
- Crispr Based Genetic Manipulation for Gut Microbiome Engineering
- Crispr/Cas9 as a Portable Diagnostic Tool for Detecting Diseases
- Conclusion
- Acknowledgements
- References
- Kriti Ghatani, Shankar Prasad Sha, Subarna Thapa, Priya Chakraborty and Sagnik
- Sarkar
- Introduction
- Origin of Bifidobacteria
- From Human Gut
- Bifidobacteria Isolated from Breast Milk
- From Feces
- From Human Vagina
- Bifidobacterium as Probiotics
- Health Benefits of Bifidobacteria
- Alleviation of Obesity
- Infant Health
- Enhancement of Immunity
- Vitamin Production
- Prevention of Constipation
- Anti-Bacterial Activity
- Amino Acid Production
- Gamma-Aminobutyric Acid (Gaba) Production
- Alleviation of Psychological Disorders
- Anti-Colorectal Cancer Properties
- Bifidobacterial Genome Editing
- Conclusion
- Acknowledgements
- References
- Aravind Sundararaman and Prakash M. Halami
- Introduction
- Types of Metabolites Produced by Bifidobacteria
- Vitamin Metabolism
- Sugar Metabolism
- Pro-, Pre-And Syn-Biotic Concepts
- Bacteriocins Produced by Bifidobacterium Sp.
- Bifidin
- Bifilong
- Bifidocin B
- Thermophilicin B67
- Genome Engineering of Metabolites
- Strategies to Improve the Transformation Efficiency
- Inducible Plasmid Self-Destruction (Ipsd) Assisted Genome Engineering
- Study on Probiotic Bacteria-Host Interactions by Genome Editing
- Bioengineer Tailored Probiotics by Genome Editing
- M Edical Applications for Bifidobacterial Gene Expression Systems
- Mutagenesis of Bifidobacterial Genes
- Conclusion
- Acknowledgements
- References
- ,Md Minhajul Abedin, Srichandan Padhi, Rounak Chourasia, Loreni Chiring Phukon
- Puja Sarkar, Sudhir P. Singh and Amit Kumar Rai
- Introduction
- Popular Lab Used in Food
- Fermented Plant Based Food Product
- Fermented Animal Based Food Product
- Lab as Starter Cultures and Their Selection
- Characterization of Lab Genomes
- Different Sequencing Approaches
- Single Cell Genomics
- Metagenomics
- Practical Insights into Lab Genomes
- Β-Galactosidase
- Antimicrobial Agents
- Bile Salt Hydrolase
- Exoploysaccharides
- Gamma-Aminobutyric Acid
- Applications of Genome Editing in Lab
- Future Directions and Conclusion
- Acknowledgements
- References
- Sudeepa E. S. And A. Sajna
- Introduction
- Lactiplantibacillus Plantarum
- Morphology
- Genome
- Metabolism
- L. Plantarum Fermented Vegetable Products
- Properties of Plant-Based Food Products Fermemted by L.
- Plantarum
- Taste/Flavour Attributes
- Exopolysaccharides Production
- Bacteriocin Production
- Probiotic and Health Benefits
- Applications of Genome Editing in L. Plantarum
- Future Prospects
- Conclusion
- Acknowledgements
- References
- Steji Raphel and Prakash M. Halami
- Introduction
- Characteristics of Bacillus Spp.
- Genomics of Bacillus Licheniformis
- General Genomic Features
- Metabolic Activity and Extracellular Enzymes
- Export Proteins
- Secondary Metabolites
- Stress Response
- Genome Editing in Bacillus Licheniformis
- Genome Editing Tools Developed in Bacillus Licheniformis
- Inducible Promoter Based Genome Engineering
- Pyrimidine Metabolism Based Genome Engineering
- Transconjugation Based Plasmid System
- Site Specific Recombination Techniques
- Flp/Frt System
- Crispr/Cas Systems
- Crispr Interference System
- Applications of Genome Editing Techniques
- Conclusion
- Acknowledgements
- References
- Subject Index
Author
- Pra kash M. Halami
- Aravind Sundararaman
