Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging “CRISPR/Cas9” technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach.
This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases.
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Table of Contents
1. History and discovery of genome engineeringArzoo Ahad, Mahnoor Ilyas, Manal Arshad, Tuba Sharf Batool, Alvina Gul, and Mahnoor Ejaz
2. A new insight into genome editing: CRIPR-Cas system in bacteria from evolution to application
Sidra Anwar, Alvina Gul, and Rumeza Hanif
3. CRISPR-Cas9-mediated genome editing in fungi: Current scenario and future implications in agriculture, health, and industry
Noor-ul-Ain Malik, Attia Rubab Khalid, Alvina Gul, Faiza Munir, Ghulam Haider, and Muhammad Faraz Bhatti
4. High-throughput genome engineering: Use of CRISPR/Cas9 in Arabidopsis thaliana modification
Manal Arshad, Tuba Sharf Batool, Sarah Fatima, Arzoo Ahad, Alvina Gul, Anum Khursheed, Muhammad Jamil, and Sami Ullah Jan
5. Genome engineering in banana
Sidra Fatima, Zeeshan Zafar, Arzoo Ahad, Alvina Gul, and Hadi Bux
6. Genetic engineering in tomato
Sana Tariq, Alvina Gul, Stefano Negri, and Tahir Mujtaba
7. Genetic engineering techniques in potato
Muhammad Abu Bakar Zia, Muhammad Ijaz, Sami-ul-Allah, Roohi Aslam, Muhammad Naeem, Alvina Gul, Razmia Sabahat Butt, and Muhammad Tahir
8. Genomic engineering in peanut
Ahmad Ali, Rabia Amir, Alvina Gul, Faiza Munir, Kainat Ahmad, and Anum Akram
9. Genome engineering in soybean
Khola Rafique, Khadija Ahmed, Kiran Kainat Sherin, Shahrukh Khan, Sana Tariq, Alvina Gul, and Madiha Gohar
10. Genome editing techniques and mechanism of canola editing
Faiza Abbas, Iqra Siddique, Alvina Gul, and Sami Ullah Khan
11. Genome engineering in wheat against drought stress
Mahnoor Ilyas, Arzoo Ahad, and Alvina Gul
12. Genome engineering in maize using CRISPR/CAS9 system
Khola Rafique, Alvina Gul, Namrah Ahmad, and Nida Mushtaq
13. Genome engineering in barley
Hajra Maqsood, Arzoo Ahad, Somia Khan, Alvina Gul, Midhat Mehboob, Resham Shaukat, and Muhammad Jamil
14. Genome engineering in rice
Amnah Asghar, Anum Akram, Nida Mushtaq, Noor-ul-Ain Malik, and Alvina Gul
15. Genome engineering in sorghum
Maria Gilani, Qurat-ul-ain Ali, Faiza Munir, Sarah Fatima, Alvina Gul, Rabia Amir, Muhammad Ammar Ashar, and Hafiz Imran Fakhar
16. Genome engineering in cotton (Gossypium hirsutum)
Maria Sharif, Uzma, Maria Abdul Salam, Afsheen Malik, Shahrukh Khan, Kiran Kainat Sheerin, Rabia Amir, Sami Ullah Jan, Alvina Gul, and Muhammad Jamil
17. Genome engineering in sugarcane
Qurat-ul-ain Ali, Faiza Munir, Saba Azeem, Alvina Gul, and Khadija Ahmed
18. Genome modifications in cassava
Hajira Imran, Danish Ilyas Baig, Warda Jabbar, Alvina Gul, Warda Gul, Munir Ozturk, Bengu Turkyilmaz Unal, and Pedro Garcia-Caparros
19. Genetic engineering on tobacco
Oushna Fajer, Alvina Gul, Isra Farooq, Nabia Khan, Maliha Fatima, Mahnoor Ilyas, and Hadi Alipour
20. Industrial applications of genetic engineering
Rabia Amir, Qurat ul ain Sani, Anum Akram, Zeeshan Zafar, and Sidra Fatima
21. The secluded economic impact of genetically modified crops
Alishbah Gul, Sobia Asghar, Muhammad Jamil, Ahsan-ul-Haq Satti, Zubair Hafeez, Usman Masood, Adnan Mazhar, Summiya Iqbal, Rao Muhammad Asad, Tanveer Hussain, and Sundas Iftikhar
22. Genome engineering and bioethics
Kiran Khurshid, Rabia Amir, Alvina Gul, Wajahat Maqsood, M. Awais Junaid, Faiq M. Sabih, and Hina Javed
Authors
Alvina Gul Associate Professor, National University of Sciences and Technology (NUST), Islamabad, Pakistan.Dr. Alvina Gul is a Tenured Associate Professor at the National University of Sciences and Technology (NUST), Islamabad, Pakistan. She previously worked for Cornell University in New York, USA; the USDA Genotyping and Sequencing Laboratory at Kansas State University; and the Plant Breeding Institute at the University of Sydney, Australia. The primary focus of her research career has been on integrating new allelic variation for improved wheat crop production and yield maximization in order to enhance food security. Dr. Gul has published more than 80 peer-reviewed publications and 140 book chapters, as well as one book with Elsevier.