Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.
Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).
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Table of Contents
1. Detection of myeloid-derived suppressor cells by flow cytometryTianju Liu
2. Determination of murine myeloid-derived suppressor cells by flow cytometry
Hiroshi Kitamura
3. Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions
Gene Man
4. Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood
Gabriel Cabrera
5. CD4+ T cells proliferation assays to analyze Mo-MDSC suppressive function
Marcos L�pez-Hoyos
6. Assessment of myeloid-derived suppressor cell differentiation ex vivo
Ester Blanco
7. Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo
Daniel Gallego-P�rez
8. Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes
Ali Syed Arbab
9. Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells
Ting Zhao and Hong Du
10. In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells
KYU HWAN KWACK and Keith Lough Kirkwood
11. Estimating Nitric Oxide (NO) from MDSCs by Griess Method
Saikat Mukherjee
12. In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs)
Kevin Alicea-Torres
