This training program will help attendees understand the sources of mycoplasma contamination and how to minimize the occurrence of contamination. The regulatory standards for testing will be described as well as the methods of mycoplasma detection and the resources for testing. At the conclusion of this webinar, attendees will have knowledge of the importance of mycoplasma contamination testing and how to successfully perform the tests in their company’s setting.
What is mycoplasma contamination?
What is the impact of mycoplasma contamination on the development and manufacture of biologics?
What are the U.S. and international regulatory standards for mycoplasma testing?
How to control mycoplasma contamination?
What are the methods of detection of mycoplasma contamination?
What are the resources needed for mycoplasma testing?
How can you ensure elimination of mycoplasma from infected cultures?
This webinar will address these core questions around the subject and more.
Why Should You Attend:
If you use continually passaged cell lines for the production of biologics in a pharmaceutical or biotechnology setting and you do not currently test for mycoplasma contamination, awareness for the need for this testing of contamination should not be ignored or overlooked. It is estimated that 15-35% of continually cultured cell lines are contaminated with mycoplasmas. Mycoplasma contamination of a cell line does not kill the host cell, but adversely affects metabolism and slows growth. Mycoplasmas are bacteria of very small size that cannot be removed by filtration and are resistant to most antibiotics. For these reasons, controlling and testing of mycoplasma contamination of continually passaged cell lines used in the development and manufacture of biologics is paramount.What is mycoplasma contamination?
What is the impact of mycoplasma contamination on the development and manufacture of biologics?
What are the U.S. and international regulatory standards for mycoplasma testing?
How to control mycoplasma contamination?
What are the methods of detection of mycoplasma contamination?
What are the resources needed for mycoplasma testing?
How can you ensure elimination of mycoplasma from infected cultures?
This webinar will address these core questions around the subject and more.
Areas Covered in the Webinar:
Background
- What is mycoplasma
- Number of mycoplasma species
Need for Testing of Mycoplasma Infection
- Effects of contamination of cell lines
- Sources of infection
- Types of test samples in biological and pharmaceutical manufacturing
- U.S. and international regulatory standards
- Methods of Detection
- Direct culturing
- Indicator cell co-cultivation
- PCR
- Resources for Detection
- In-lab with commercial kits
- Commercial lab services
Mycoplasma elimination and prevention
- Methods of eliminating mycoplasma contamination
- Processes to prevent mycoplasma contamination
Who Will Benefit:
This webinar will benefit those in the pharmaceutical, biotechnology and medical device industries that are using continually passaged cell lines for production of biologics and are not currently testing for mycoplasma contamination.- Research and development managers, supervisors and technicians
- Manufacturing managers, supervisors and technicians
- Quality control managers, supervisors and technicians
- Senior management
Course Provider
Michael Simonian,
