Extracellular Vesicles for Therapeutic and Diagnostic Applications provides an introduction to exosomes and extracellular vesicles, including their unique properties and characterization, before detailing the most-utilized isolation, purification, and surface engineering techniques for their preparation as therapeutic and diagnostic agents. This book reviews recent developments in interdisciplinary research on exosome structure, properties, and engineering approaches as well as their use in diagnosis and treatment of a variety of illnesses such as cancer, tuberculosis, Alzheimer's disease, ophthalmic diseases, and others. Extracellular Vesicles for Therapeutic and Diagnostic Applications is an important reference for those interested in the development and application of exosomes and extracellular vesicles as biomaterials for us in the diagnosis and treatment of disease.
Table of Contents
1. Overview of extracellular micro and nano vesicles 2. Biogenesis metastatic formation of exosomes and other extracellular vesicles 3. Overview on Characterization of Extracellular Vesicles 4. Exosomes and other extracellular vesicles -mediated cell-cell communication 5. Isolation and purification of exosomes and other extracellular vesicles 6. Detection and quantification of exosomes and other extracellular vesicles 7. Characterization of exosomes and other extracellular vesicles 8. Exosomes and other extracellular vesicles for diagnostics 9. Exosomes and other extracellular vesicles: preclinical studies 10. Exosomes and other extracellular vesicles for cancer management 11. Exosomes and other extracellular vesicles for cardiovascular diseases 12. Exosomes and other extracellular vesicles for neurogenerative diseases 13. Exosomes and other extracellular vesicles for infectious diseases 14. Exosomes and other extracellular vesicles for bone diseases 15. Exosomes and other extracellular vesicles for reproductive medicines 16. Exosomes and other extracellular vesicles for ophthalmic and dermal ailments 17. Exosomes and other extracellular vesicles-based vaccine delivery 18. Exosomes and other extracellular vesicles in regenerative medicines 19. Exosomes and other extracellular vesicles in gene therapy and personalized care 20. Bioinformatics, AI and ML for exosomes and other extracellular vesicles 21. Regulatory concerns for exosomes and extracellular vesicles 22. Future perspectives and challenges of extracellular vesicles in therapeutics and diagnostics
Authors
Anand Krishnan Department of Chemical Pathology, School of Pathology, Office of the Dean, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa..
Dr. Anand Krishnan is a Principal Investigator in the Department of Chemical Pathology, School Pathology, Office of the Dean, Faculty of Health Sciences, University of the Free State, South Africa. He leads the Precision Medicine Integrative Nano Diagnostics (P-MIND) laboratories, which focus on the biological functions of extracellular vesicles (EVs) in synthetic biology. His research encompasses cell surface protein modification, biomarker discovery through EVs derived from liquid biopsies, and the role of circulating biomarkers in disease development and stem cell biology. Dr. Krishnan has authored over 150 articles and edited eight books published by Elsevier, Springer, and Wiley. He received the National Research Foundation (NRF) rating for Next Generation Researchers in South Africa. In the latest list published by Stanford University, Dr. Krishnan was named among the Top 2% of the most influential scientists worldwide. He is an active member of the International Society of Extracellular Vesicles (ISEV), the African Organisation for Research and Training in Cancer (AORTIC), and the American Association of Cancer Research (AACR). Recently, he has been serving as an editor for the eLife journal, focusing on the Africa region.
Vadivalagan Chithravel Department of Surgery, Michigan Medicine, University of Michigan Medical Centre, Ann Arbor, Michigan, USA.
Dr. Vadivalagan Chithravel works as a Research Fellow in the Department of Surgery at the University of Michigan, Ann Arbor, specializing in medical science, excluding epidemiology. The Exosome Mediated Signal Transduction Mechanism is his area of expertise. His current work focuses on a glycosylation biomarker associated with an exosome pathway for the physiology of cells with HCC. In addition to being a group director and scientist at the SAARP foundation in India, he has held postdoctoral research fellow positions at Taipei Medical University and China Medical University in Taiwan. He is also an active member of the International Society of Extracellular Vesicles and the American Association of Extracellular Vesicles.
Prakash Gangadaran Department of Biomedical Science and Nuclear Medicine. School of Medicine, Kyungpook National University, Jung-gu, Daegu, South Korea.
Dr. Prakash Gangadaran is Principal Investigator and FOUR-Brain Korea Post-Doctoral Fellow at the Department of Biomedical Science and Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea. He has expertise in utilization of extracellular vesicles in angiogenesis, wound healing, drug delivery, molecular and nuclear imaging. He has published more than 48 research articles in international peer-reviewed journals and has authored 2 book chapters as well as 24 review articles. His research interests include extracellular vesicles, angiogenesis, wound healing and drug delivery, radiotherapies, molecular imaging and nuclear imaging.
Sathish Muthu Department of Orthopaedics, Government Medical College, Karur, Tamil Nadu, India.
Dr. Sathish Muthu is an Assistant Professor in the Department of Orthopaedics at Government Medical College, Karur, India. He is the Co-Founder and Head of Research at Orthopaedic Research Group, a non-profit research organization dedicated to translational orthopaedic research. He is also a steering committee member of the Indian Stem Cell Study Group. and associate member of Knowledge Forum Degenerative of AO Spine. Despite being a board-certified orthopaedic surgeon, Dr. Muthu holds fellowships in spine surgery, orthopaedic rheumatology, and regenerative medicine. He has published over 275 articles in international peer-reviewed journals and has contributed 5 chapters to books on cartilage injury and regeneration. He is also an Associate Editor at various academic journals like PLOS One, Journal of Clinical Medicine, Indian Journal of Orthopaedics, Journal of Orthopaedic Case Reports. His research focuses on cartilage biology, mesenchymal stem cells, exosomes, degenerative disorders, osteoarthritis, and cartilage regeneration.
Ben Peacock Head of Research. NanoFCM Co., Ltd, Nottingham, UK.
Dr. Ben Peacock is Head of Research at NanoFCM Co., Ltd. He has expertise in exosomal miRNA and protein profiles of EVs from cancer cell lines by RNA-seq and mass spectrometry (ion-trap) with validation by RT-qPCR and further western blotting. In international peer-reviewed journals, he has published more than nine research articles. His research interests include EV research, but he is well established in stromal communication of cancers of the head and neck. Multiple procedures were optimized to maximize EV generation from cell lines, improve concentration and purity of EV isolations, and implement best practices for sample storage.