Immuno-Oncology And Immunotherapy - Part A Volume 189, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include, Generation of transmitochondrial cybrids in cancer cells, Methods for flow cytometry analysis for tumor infiltrating cells functions and phenotype, Innovative 2D and 3D methods of tumor cells culture, and more.
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Table of Contents
1. Assessment of translation rate in leukemic cells and immune cells of the microenvironment by OPP protein synthesis assay
Vanessa Klapp, Ozgu Gumustekin, J�r�me Paggetti, Etienne Moussay and Anne Largeot
2. Generation of transmitochondrial cybrids in cancer cells
Ruth Soler-Agesta, Cristina Ripoll�s-Yuba, Joaqu�n Marco-Brualla, Raquel Moreno-Loshuertos, Ai Sato, Manuel Beltr�n-Visiedo, Lorenzo Galluzzi and Alberto Anel
3. Enzyme-linked Immunospot (Elispot) assay to quantify peptide-specific IFN-? production by splenocytes in a mouse tumor model after radiation therapy
Beno�t Lecoester, Yaoyao Xie, Am�lie Marguier, Laura Boulerot, Marine Malfroy, Olivier Adot�vi and Jihane Boustani
4. Tumor slice culture system for ex vivo immunotherapy studies
Leire Arrizabalaga, Joan Salvador Russo-Cabrera, Virginia Bels�e, Pedro Berraondo, Ignacio Melero and Fernando Aranda
5. Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells
Alberto Susana, Giovanni Galletti, Gabriele De Simone, Chiara Camisaschi and Enrico Lugli
6. Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors
Vladim�r Kouck�, Linn A. Syding, Kl�ra Plackov�, Lucie Pavelkov� and Anna Fialov�
7. Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids
Ainara Lopez-Pardo, Ainhoa Amarilla-Irusta, V�ctor Sand�, Mario Stan, Francisco Borrego and Laura Amo
8. Characterization of Natural Killer cells infiltrating irradiated murine tumors through flow cytometry
J�r�my Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot, V�ronique Morgand and C�line Mirjolet
9. Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation
Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable
10. Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line
Ginevra Campia, Manuel Beltr�n-Visiedo, Ruth Soler-Agesta, Ai Sato, Norma Bloy, Liwei Zhao, Peng Liu, Oliver Kepp, Guido Kroemer, Lorenzo Galluzzi and Claudia Galassi
11. Optimization and intra-assay validation of a multiparametric flow cytometric test for monitoring circulating TREGs
Iole Macchia, Floriana Iacobone and Francesca Urbani
12. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow
Manuel Beltr�n-Visiedo, Alfonso Serrano-Del Valle, Nelia Jim�nez-Ald�an, Ruth Soler-Agesta, Javier Naval, Lorenzo Galluzzi and Isabel Marzo
