Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
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Table of Contents
1. PrefaceCharpentier Maud and Norma Bloy
2. Tumor slice culture system for ex vivo immunotherapy studies
Fernando Aranda
3. Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids
Francisco Borrego
4. ELISpot assay to quantify peptide-specific IFN? production by splenocytes in a mouse tumor model of radiation therapy
Jihane Boustani
5. Flow cytometry detection and quantification of circulating leucocyte subpopulations in mice after brain irradiation
Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable
6. Assessment of pDC functional capacity upon exposure to tumor-derived soluble factors
Anna Fialov�
7. Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line
Lorenzo Galluzzi
8. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow
Lorenzo Galluzzi
9. Generation of transmitochondrial cybrids in cancer cells
Lorenzo Galluzzi
10. Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells
Enrico Lugli, Alberto Susana, Giovanni Galletti, Gabriele De Simone and Chiara Camisaschi
11. Optimization and validation of a multiparametric flow cytometric assay for monitoring peripheral blood TREGs
Iole Macchia Sr., Floriana Iacobone and Francesca Urbani
12. Characterization of NK cells infiltrating irradiated murine tumors through flow cytometry
C�line Mirjolet, J�r�my Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot and V�ronique Morgand
13. Assessment of translation rate in leukemic cells and immune cells of the tumor microenvironment by OPP protein synthesis assay
Jerome Paggetti, Vanessa Klapp, Anne Largeot, Ozgu Gumustekin and Etienne Moussay
