Immuno-oncology and immunotherapy, Part B, Volume 191 in the Methods in Cell Biology series, highlights new advances, with this volume presenting chapters on a variety of topics, including Multiparametric flow cytometry study of human MDSCs, In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance, Machine learning approach to assess brain metastatic burden in preclinical models, Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death, 3D agar colony inhibition assays to functionally evaluate HER2-targeting antibodies against human cancer cells elicited by vaccination in mice, and much more.
Additional sections cover Spatial immunophenotyping of FFPE tissues by imaging mass cytometry, Processing human colon cancer specimens for in vitro cytotoxicity assays, A xenograft model of metastatic colon cancer, A preclinical model of hepatic metastasis to instruct effective treatment modalities, Myeloid-derived suppressor cells: Identification and function, Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer, and Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse.
Additional sections cover Spatial immunophenotyping of FFPE tissues by imaging mass cytometry, Processing human colon cancer specimens for in vitro cytotoxicity assays, A xenograft model of metastatic colon cancer, A preclinical model of hepatic metastasis to instruct effective treatment modalities, Myeloid-derived suppressor cells: Identification and function, Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer, and Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse.
Table of Contents
1. Multiparametric flow cytometry study of human MDSCsLuis de la Cruz-Merino and Victor Sanchez-Margalet Sr.
2. In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance
Yanfeng Gao, Zhuouying He, Xiuman Zhou and Youmei Xiao
3. Machine learning approach to assess brain metastatic burden in preclinical models
Romina Goldszmid
4. Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death
Dmitri Krysko, Faye Naessens, Iuliia Efimova and Mariia Saviuk
5. 3D agar colony inhibition assays to functionally evaluate HER2-targeting antibodies against human cancer cells elicited by vaccination in mice
Pier-Luigi Lollini, Francesca Ruzzi, Maria Sofia Semprini, Laura Scalambra, Stefania Angelicola, Chiara Cappello, Olga Maria Pittino, Patrizia Nanni and Arianna Palladini
6. Spatial immunophenotyping of FFPE tissues by imaging mass cytometry
Barbara Maier
7. Processing human colon cancer specimens for in vitro cytotoxicity assays
Sofia Mensurado Santos, Rafael Blanco Dom�nguez, Bruno Silva-Santos, Mariana Carreira and Leandro Barros
8. A xenograft model of metastatic colon cancer
Sofia Mensurado Santos and Rafael Blanco Dom�nguez
9. A preclinical model of hepatic metastasis to instruct effetive treatment modalities
Anna Mondino, Citro Antonio, Lorenzo Piemonti, Arianna Pocaterra, Chiara Gnasso, Tamara Canu, Anna Tosi, Antonio Rosato and Antonio Esposito
10. Myeloid-derived suppressor cells: Identification and function
Lorenzo Moretta, Nicola Tumino, Maria Teresa Bilotta and Paola Vacca
11. Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer
Marcus Ruscetti, Shreya Chowdhury, Katherine Murphy, Chaitanya Parikh, Kelly DeMarco and Lin Zhou
12. Selective expansion of anti-tumour innate lymphocytes in long-term cultures after a single BCG pulse
Mar Vales-Gomez
