Immuno-Oncology And Immunotherapy - Part B Volume 190, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. In this update, chapters include Machine learning methods to assess metastatic burden, Development of pre-clinical models, Tumor microenvironment analysis by multiparametric flow cytometry or/and mass cytometry, Measure of therapy induced cell senescence, and much more.
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Table of Contents
1. Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling
Roc�o Flores-Campos, Daniel J. Garc�a-Dom�nguez, Lourdes Hontecillas-Prieto, Carlos Jim�nez-Cortegana, Luis de la Cruz-Merino and V�ctor S�nchez-Margalet
2. In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance
Zhuoying He, Xiuman Zhou, Youmei Xiao and Yanfeng Gao
3. Machine learning approach to assess brain metastatic burden in preclinical models.
Jessica Rappaport, Quanyi Chen, Tomi McGuire, Am�lie Daugherty-Lop�s and Romina Goldszmid
4. Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death
Faye Naessens, Iuliia Efimova, Mariia Saviuk and Dmitri V. Krysko
5. Functional evaluation of circulating anti-cancer antibodies with a 3D tumor cell growth inhibition assay
Francesca Ruzzi, Maria Sofia Semprini, Laura Scalambra, Stefania Angelicola, Chiara Cappello, Olga Maria Pittino, Patrizia Nanni, Arianna Palladini and Pier-Luigi Lollini
6. Spatial immunophenotyping of FFPE tissues by imaging mass cytometry
Carolina Mangana and Barbara B. Maier
7. Processing human colon cancer specimens for in vitro cytotoxicity assays
Sofia Mensurado, Rafael Blanco-Dom�nguez, Mariana Carreira, Leandro Barros and Bruno Silva-Santos
8. An orthotopic metastatic xenograft model of colorectal cancer
Rafael Blanco-Dom�nguez, Sofia Mensurado, Leandro Barros, Mariana Carreira and Bruno Silva-Santos
9. A preclinical mouse model of hepatic metastasis to instruct effective treatment modalities
Arianna Pocaterra, Antonio Citro, Chiara Gnasso, Tamara Canu, Anna Tosi, Antonio Rosato, Antonio Esposito, Lorenzo Piemonti and Anna Mondino
10. Myeloid-derived suppressor cells: Identification and function
Paola Vacca, Maria Teresa Bilotta, Lorenzo Moretta and Nicola Tumino
11. Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer
Shreya R. Chowdhury, Katherine C. Murphy, Chaitanya N. Parikh, Kelly D. DeMarco, Lin Zhou and Marcus Ruscetti
12. Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse
Mar�a-Jos� Felgueres, Gloria Esteso, Nacho Aguil� and Mar Val�s-G�mez
