Practical Biochemistry provides both foundational knowledge and advanced insights into biochemistry, including the basic compounds, and laboratory methods. The book is designed for students and academic professionals seeking a comprehensive understanding of the practical aspects of the subject.
The book is systematically divided into five sections, each dedicated to a specific category of macromolecules and related biochemical techniques: 1) Carbohydrates, 2) Proteins, 3) Nucleic acids, 4) Lipids, 5) Supplementary Techniques and Safety Data Sheet (SDS). Each chapter within these sections is structured to provide a thorough understanding of the aim, principles, procedures, and practical applications of biochemical techniques.
The book is systematically divided into five sections, each dedicated to a specific category of macromolecules and related biochemical techniques: 1) Carbohydrates, 2) Proteins, 3) Nucleic acids, 4) Lipids, 5) Supplementary Techniques and Safety Data Sheet (SDS). Each chapter within these sections is structured to provide a thorough understanding of the aim, principles, procedures, and practical applications of biochemical techniques.
Key features:
- Comprehensive Information: meticulously organized and structured chapters provide a thorough and methodical approach to learning
- Additional Learning Tools: ‘Did You Know’ segments and ‘Viva Voice’ questions enrich the learning experience by offering interesting facts and stimulating critical thinking
- Practical Focus: Step-by-step guides aid readers in understanding and applying the techniques in the lab
- Safety and Accuracy: teaches how to conduct safe and accurate experiments with precautions
- Accessible Language: simple and lucid language helps beginners to understand complex biochemical concepts
Readership:
- This is a primary resource for biochemistry and other life science research courses at college and university levels that require learning practical laboratory techniques.
Table of Contents
FOREWORD- PREFACE
- LIST OF CONTRIBUTORS
- Mrittika Sarkar, Sinchan Hait and Sai Joshi
- INTRODUCTION
- Classification of Carbohydrates
- Monosaccharides (mono = one)
- Oligosaccharides (oligo = few)
- Polysaccharides (poly = many)
- GLYCOSIDIC BOND
- N-glycosides
- C-glycosides
- S-glycosides
- O-glycosides
- REDUCING AND NON-REDUCING SUGARS
- PROPERTIES OF CARBOHYDRATES
- Elemental Composition
- Physical Properties
- Stereoisomers
- Geometrical (cis-trans isomers)
- Enantiomers
- Optical Activity
- Epimers
- Anomers
- CONCLUSION
- VIVA VOCE
- REFERENCES
- Mrittika Sarkar, Sinchan Hait and Sai Joshi
- INTRODUCTION
- Solubility Test
- Aim
- Principle
- Requirements
- Procedure
- Applications
- Conclusion
- Molisch’s Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Applications
- Fehling Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Applications
- Benedict’s Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Applications
- Seliwanoff’s Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Troubleshooting
- Applications
- Bial’s Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Application
- Iodine Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Troubleshooting
- Application
- Barfoed’s Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Application
- Osazone Test
- Aim
- Principle
- Requirements
- Procedure
- Conclusion
- Precautions
- Troubleshooting
- Application
- DID YOU KNOW?
- CONCLUSION
- VIVA VOCE
- REFERENCES
- Mrittika Sarkar, Anshika Sah, Payal Agarwal, Sai Joshi and Sinchan Hait
- INTRODUCTION
- ANTHRONE METHOD
- Principle
- Hydrolysis of Complex Carbohydrates
- Dehydration of Monosaccharide
- Reaction with Reagent
- Requirements
- Procedure
- Results
- Did you know?
- DNSA METHOD
- Principle
- Requirements
- Procedure
- Results
- Did you know?
- FOLIN-WU METHOD
- Principle
- Requirements
- Procedure
- Results
- HEXOKINASE METHOD
- Principle
- Requirements
- Procedure
- Results
- PHENOL-SULFURIC ACID METHOD
- Principle
- Requirements
- Procedure
- Results
- SOMOGVI-NELSON METHOD
- Principle
- Requirements
- Procedure
- Results
- GOPOD METHOD
- Principle
- Requirements
- Procedure
- Results
- CONCLUSION
- VIVA VOCE
- REFERENCES
- Sinchan Hait and Sai Joshi
- INTRODUCTION
- PROTEIN STRUCTURE
- FUNCTIONS OF PROTEIN
- Enzymes
- Membranes and Transport Molecules
- Antibodies and Toxins
- Functional Proteins
- Structural Proteins
- CONCLUSION
- VIVA VOCE
- REFERENCES
- Sinchan Hait
- INTRODUCTION
- AROMATIC AMINO ACIDS (TYROSINE, TRYPTOPHAN)
- Xanthoproteic Test
- Aim
- Principle
- Requirement
- Procedure
- Results
- Precaution
- Application
- Limitations
- TYROSINE
- Millon’s Test
- Aim
- Principle
- Requirement
- Procedure
- Results
- Precaution
- Application
- Limitations
- TYROSINE AND HISTIDINE
- Pauly’s Diazo Test
- Aim
- Principle
- Requirement
- Procedure
- Results
