Handbook of Translational Transcriptomics: Research, Protocols and Applications provides a comprehensive overview on the field of transcriptomics. The book begins with an overview of the past, present, and potential approaches in the field of transcriptomics, with discussions on choosing the correct approach based on the research needed. The book also highlights the pros and cons of each approach and explores techniques and protocols for investigating specific avenues, focusing on RNA sequencing, expression arrays, and gene expression. Sections on data analysis offer recommendations, guidelines, and approaches related to data interpretation.
The book also considers the translation of transcriptomics to clinical application and applications in molecular diagnostics, biomarkers in medicine, and personalized medicine specific to oncology, as well as biotechnology for pharmaceutical research.
Table of Contents
1. Past, current and future of transcriptomics
2. Pitfalls of transcriptomics, and selection of the most appropriate transcriptomic technique
3. RNA sequencing in wet lab
4. Expression arrays in wet lab
5. High-throughput and multiplex PCR in wet lab to measure gene expression
6. Processing primary gene expression data: normalization, harmonization and data QC
7. Data check and comparison with the expression databases
8. Finding differentially expressed genes and gene sets
9. Molecular pathway analysis using transcriptomic data
10. OMICSwise analysis of RNA splicing
11. Transcriptomic biomarkers in medicine
12. Translational transcriptomics for personalized oncology
13. Transcriptomics for modern biotechnology
14. Transcriptomics and quantitative proteomics: competition or symbiosis?
Authors
Anton Buzdin Professor and Head of Laboratories, Sechenov First Moscow State Medical University, Moscow, Russian Federation Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation. Pathobiology Group of European Organization for Research and Treatment of Cancer (EORTC), Brussels, Belgium Omicsway Corporation, Walnut, USA. Anton Buzdin is professor and head of laboratories at Sechenov First Moscow Medical University, Moscow Institute of Physics and Technology, and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry. He was elected as the chair of Biostatistics and Bioinformatics subgroup, Pathobiology Group of European Organization for Research and Treatment of Cancer (EORTC). Professor Buzdin is cofounder and chief scientific officer of Oncobox, which is a translational oncology company. He has authored more than 170 scientific papers and patent applications, and has worked as chief executive of several biotechnological and biomedical companies in Russia, Hong Kong and in the United States. He has also chaired two clinical trials of transcriptomics-based diagnostic tools in oncology.Professor Buzdin's team pioneered bioinformatic quantitative analysis of molecular pathways using gene expression data and developed specialized algorithms and software packages to personalize prescription of targeted immunotherapy and chemotherapy drugs to cancer patients that were clinically validated and commercialized.
