Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique, including isolation techniques for DNA and RNA, gene cloning, PCR, DNA sequencing, ELISA and cell culture. These protocols all includes a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
Table of Contents
1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria
2. Isolation of Plasmid
3. Isolation of RNA
4. PCR (Polymerase Chain reaction)
5. Real-time (Quantitative) PCR
6. Electrophoresis
7. Capillary electrophoresis
8. Purification of Nucleic acid
9. Southern blotting
10. Northern blotting
11. ELISA (Enzyme Linked Immuno Sorbent Assay)
12. EMSA (Electrophoretic Mobility Shift Assay)
13. Gene Cloning
14. End labelling of DNA
15. DNA sequencing
16. RNA sequencing
17. Microarray
18. Generation of Genomic and cDNA library.
19. Western Blotting
20. Cell Culture
21. Fluorescent activated cell sorting (FACS)
22. DNA microarray
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Basics of Bioinformatics
26. Isolation of Proteins
27. Protein purification
28. Fluorescence In situ Hybridization
