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Biocatalysis. Methods in Enzymology Volume 714

  • Book

  • April 2025
  • Elsevier Science and Technology
  • ID: 6035917
Biocatalysis, Volume 714 provides a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Specific chapters in this new release include Biocatalysis: How to select the proper mode of application, Documentation in biocatalysis and data handling, Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications, Functional Metaproteomics, Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases, P450 monooxygenase in whole-cell format, and more.

Additional sections cover Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells, Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species, C.necator as a model organism for CO2-based biotechnology, Cupriavidus in whole-cell biocatalysis, Whole-cell biocatalysis with Myxobacteria, Streptomyces for natural product formation: Targeted mutagenesis in PKS, Reductive Amination: Methods for cell-free and whole-cell biocatalysis, Challenges and good practices on transaminase-catalysed synthesis of optically pure amines, W-enzymes in biocatalysis: Chances and difficulties for the user, Atroposelective biocatalysis employing ADHs, Applications of alcohol oxidases, and much more.

Table of Contents

1. Biocatalysis: How to select the proper mode of application
Dirk Tischler and Jose Munoz
2. Documentation in biocatalysis and data handling
J�rgen Pleiss
3. Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications
Jose Munoz
4. Functional Metaproteomics
Lars I. Leichert
5. Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases
Dirk Tischler
6. P450 monooxygenase in whole-cell format
Carolin M�gge
7. Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells
Rolandas Meskys
8. Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species
Maciej Szaleniec
9. C.necator as a model organism for CO2-based biotechnology
Lars Lauterbach and Sandy Schmidt
10. Cupriavidus in whole-cell biocatalysis
Sandy Schmidt
11. Whole-cell biocatalysis with Myxobacteria
Markus Nett
12. Streptomyces for natural product formation: Targeted mutagenesis in PKS
Frank Schulz
13. Reductive Amination: Methods for cell-free and whole-cell biocatalysis
Francesco G. Mutti, Vasilis Tseliou, Matteo Damian, Josemarco Mendoza-Avila and Marco Rabuffetti
14. Challenges and good practices on transaminase-catalysed synthesis of optically pure amines
Ioannis V. Pavlidis
15. W-enzymes in biocatalysis: Chances and difficulties for the user
Peter-Leon Hagedoorn
16. Atroposelective biocatalysis employing ADHs
Gonzalo de Gonzalo
17. Applications of alcohol oxidases
Juan Mangas-S�nchez
18. Cascades with Oxidoreductases: Enzyme and Chemistry
Caroline Emilie Paul
19. In vitro biocatalysis with P450 monooxygenases
Pimchai Chaiyen
20. Construction of artificial fusion proteins for biocatalysis
Anna Christina Ngo
21. Production and engineering of chimeric UPOs
Miguel Alcalde
22. Applications of UPOs
Frank Hollmann
23. Cell free protein synthesis for biocatalysis
Katrin Rosenthal
24. Immobilization for a stable biocatalyst application
Katarzyna Szymanska, Karolina Labus, Aleksandra Lambarska and Katarzyna Czyzewska
25. Assessment of biocatalytic processes
Stephan L�tz
26. Assay development for PET degradation by biocatalysts
�lvaro Lorente-Ar�valo and Aurelio Hidalgo