The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM)
The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope - giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth.
Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more.
- Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value
- Provides insight into the design and development philosophy behind current instrument manufacturers
- Covers sample handling, applications, and key supporting techniques
- Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research
- Presented in full colour
An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy - specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Table of Contents
About the Editors xix
List of Contributors xxi
Foreword xxv
1 Scanning Electron Microscopy: Theory, History and Development of the Field Emission Scanning Electron Microscope 1
David C. Joy
2 Akashi Seisakusho Ltd - SEM Development 1972-1986 7
Michael F. Hayles
3 Development of FE-SEM Technologies for Life Science Fields 25
Mitsugu Sato, Mami Konomi, Ryuichiro Tamochi and Takeshi Ishikawa
4 A History of JEOL Field Emission Scanning Electron Microscopes with Reference to Biological Applications 53
Kazumichi Ogura and Andrew Yarwood
5 TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy 79
Jaroslav Jiruše, Vratislav Košˇtál and Bohumila Lencová
6 FEG-SEM for Large Volume 3D Structural Analysis in Life Sciences 103
Ben Lich, Faysal Boughorbel, Pavel Potocek and Emine Korkmaz
7 ZEISS Scanning Electron Microscopes for Biological Applications 117
Isabel Angert, Christian Böker, Martin Edelman, Stephan Hiller, Arno Merkle and Dirk Zeitler
8 SEM Cryo-Stages and Preparation Chambers 143
Robert Morrison
9 Cryo-SEM Specimen Preparation Workflows from the Leica Microsystems Design Perspective 167
Guenter P. Resch
10 Chemical Fixation 191
Bruno M. Humbel, Heinz Schwarz, Erin M. Tranfield and Roland A. Fleck
11 A Brief Review of Cryobiology with Reference to Cryo Field Emission Scanning Electron Microscopy 223
Roland A. Fleck, Eyal Shimoni and Bruno M. Humbel
12 High-Resolution Cryo-Scanning Electron Microscopy of Macromolecular Complexes 265
Sebastian Tacke, Falk Lucas, Jeremy D. Woodward, Heinz Gross and Roger Wepf
13 FESEM in the Examination of Mammalian Cells and Tissues 299
Andrew Forge, Anwen Bullen and Ruth Taylor
14 Public Health/Pharmaceutical Research - Pathology and Infectious Disease 311
Paul A. Gunning and Bärbel Hauröder
15 Field Emission Scanning Electron Microscopy in Cell Biology Featuring the Plant Cell Wall and Nuclear Envelope 343
Martin W. Goldberg
16 Low-Voltage Scanning Electron Microscopy in Yeast Cells 363
Masako Osumi
17 Field Emission Scanning Electron Microscopy in Food Research 385
Johan Hazekamp and Marjolein van Ruijven
18 Cryo-FEGSEM in Biology 397
Paul Walther
19 Preparation of Vitrified Cells for TEM by Cryo-FIB Microscopy 415
Yoshiyuki Fukuda, Andrew Leis and Alexander Rigort
20 Environmental Scanning Electron Microscopy 439
Rudolph Reimer, Dennis Eggert and Heinrich Hohenberg
21 Correlative Array Tomography 461
Thomas Templier and Richard H.R. Hahnloser
22 The Automatic Tape Collection UltraMicrotome (ATUM) 485
Anwen Bullen
23 SBEM Techniques 495
Christel Genoud
24 FIB-SEM for Biomaterials 517
Lucille A. Giannuzzi
25 New Opportunities for FIB/SEM EDX in Nanomedicine: Cancerogenesis Research 533
Damjana Drobne, Sara Novak, Andreja Erman and Goran DraÂi´c
26 FIB-SEM Tomography of Biological Samples: Explore the Life in 3D 545
Caroline Kizilyaprak, Damien De Bellis, Willy Blanchard, Jean Daraspe and Bruno M. Humbel
27 Three-Dimensional Field-Emission Scanning Electron Microscopy as a Tool for Structural Biology 567
J.D. Woodward and R.A. Wepf
28 Element Analysis in the FEGSEM: Application and Limitations for Biological Systems 589
Alice Warley and Jeremy N. Skepper
29 Image and Resource Management in Microscopy in the Digital Age 611
Patrick Schwarb, Anwen Bullen, Dean Flanders, Maria Marosvölgyi, Martyn Winn, Urs Gomez and Roland A. Fleck
30 Part 1: Optimizing the Image Output: Tuning the SEM Parameters for the Best Photographic Results 625
Oliver Meckes and Nicole Ottawa
31 A Synoptic View on Microstructure: Multi-Detector Colour Imaging, nanoflight® 659
Stefan Diller
Index 679
