New Approaches for Flavin Catalysis, Volume 620, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this update include Anaerobiosis and Methods for Reduction, Reduction Potentials, Anaerobic Stopped-Flow, No Glove-Box, Anaerobic Stopped-Flow, in a Glove-Box, Chemical Quenching, Oxygen Reactions, Double-mixing Stopped-Flow, Kinetic Isotope Effects and Viscosity Effects, Heavy Enzymes Synthetic Flavins & Linear Free Energy Relationships, Vibrational Spectroscopy, Stark Spectroscopy, EPR and Related Methods, Molecular Dynamics, Phylogenetic Relationships/Superfamilies, O2 and Superoxide Analogs, and more.
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Table of Contents
1. Methods for determining the reduction potentials of flavin enzymes Shelbi L. Christgen, Sophia M. Becker and Donald F. Becker 2. Anaerobic methods for the transient-state study of flavoproteins: The use of specialized glassware to define the concentration of dioxygen Graham R. Moran 3. Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber Hannah Valentino and Pablo Sobrado 4. Chemical quenching and identification of intermediates in flavoenzyme-catalyzed reactions Kalani Karunaratne and Tatiana V. Mishanina 5. On the use of noncompetitive kinetic isotope effects to investigate flavoenzyme mechanism Kevin Francis and Giovanni Gadda 6. Isotopically labeled flavoenzymes and their uses in probing reaction mechanisms Andreea I. Iorgu, Matthew J. Cliff, Jonathan P. Waltho, Nigel S. Scrutton and Sam Hay 7. Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships Christopher J. Thibodeaux, Wei-chen Chang and Hung-wen Liu 8. Vibrational spectroscopy of flavoproteins James N. Iuliano, Jarrod B. French and Peter J. Tonge 9. Measuring electronic structure properties of flavins and flavoproteins by electronic stark spectroscopy Robert J. Stanley and Cornelius van Galen 10. EPR spectroscopy on flavin radicals in flavoproteins Daniel Nohr, Stefan Weber and Erik Schleicher 11. Applications of molecular modeling to flavoproteins: Insights and challenges Emil Sjulstok, Ilia A. Solov'yov and Peter L. Freddolino 12. Exploring the sequence, function, and evolutionary space of protein superfamilies using sequence similarity networks and phylogenetic reconstructions Janine N. Copp, Dave W. Anderson, Eyal Akiva, Patricia C. Babbitt and Nobuhiko Tokuriki 13. Structural methods for probing the interaction of flavoenzymes with oxygen and its surrogates Raspudin Saleem-Batcha and Robin Teufel 14. Reduction midpoint potentials of bifurcating electron transfer flavoproteins Anne-Frances Miller, H. Diessel Duan, Taylor A. Varner and Nishya M. Raseek 15. Investigations of two-component flavin-dependent monooxygenase systems John M. Robbins and Holly R. Ellis 16. The styrene monooxygenase system George T. Gassner 17. Flavin-N5-oxide intermediates in dibenzothiophene, uracil, and hexachlorobenzene catabolism Sanjoy Adak and Tadhg P. Begley 18. Prenylated FMN: Biosynthesis, purification, and Fdc1 activation Anna N. Khusnutdinova, Johnny Xiao, Po-Hsiang Wang, Khorcheska A. Batyrova, Robert Flick, Elizabeth A. Edwards and Alexander F. Yakunin 19. Heterologous production, reconstitution and EPR spectroscopic analysis of prFMN dependent enzymes Stephen Marshall, Karl A.P. Payne, Karl Fisher, Deepankar Gahloth, Samuel S. Bailey, Arune Balaikaite, Annica Saaret, Irina Gostimskaya, Godwin Aleku, Huanming Huang, Stephen E.J. Rigby, David Procter and David Leys 20. Physical methods for studying flavoprotein photoreceptors Estella F. Yee, Siddarth Chandrasekaran, Changfan Lin and Brian R. Crane
