Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more.
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Table of Contents
1. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage Michael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger 2. Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension Daniel C. Scott and Brenda A. Schulman 3. A genetic approach to study polyubiquitination in Saccharomyces cerevisiae Fernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski 4. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins Viduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden 5. Methods to measure ubiquitin chain length and linkage Fumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki 6. Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka 7. Methods to study phosphoribosylated ubiquitin ligation and removal Jiazhang Qiu and Zhao-Qing Luo 8. Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assays Nathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler 9. Methods to study SUMO dynamics in yeast Stefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen 10. Approaches for investigating the extracellular signaling function of ISG15 Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse 11. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10 Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup 12. Methods to analyze STUbL activity Emma Branigan, Anna Plechanovová and Ronald T. Hay 13. Quantitative analysis of USP activity in vitro Shreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma 14. Evaluating enzyme activities and structures of DUBs Jonathan N. Pruneda and David Komander 15. Purification and functional characterization of the DUB domain of SdeA Kedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das 16. Profiling DUBs and Ubl-specific proteases with activity-based probes Paul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa 17. Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues Kathrin Kunz, Stefan Müller and Luca Mendler
