Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.
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Table of Contents
- Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substratesOphelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes
- Chemical tools for selective activity profiling of bacterial penicillin-binding proteins
Shabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson
- Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking
Samir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel
- Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes
Nihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan
- Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations
Andrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright
- Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity
Byungchul Kim, Juliel Espinosa and Howard C. Hang
- Controlled release of bioactive signaling molecules using DNA-icosahedron
Maulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan
- Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation
Hui Chen and Matthew C. Good
- Engineering reversible cell-cell interactions with chemical biology
Clifford M. Csizmar and Carston R. Wagner
- Fast phosphine-activated control of protein function using unnatural lysine analogues
Joshua S. Wesalo and Alexander Deiters
- Photopharmacological control of lipid function
Johannes Morstein and Dirk Trauner
- Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targets
Miguel Macias-Contreras, Kevin N. Little and Lei Zhu
- Light-induced protein proximity by activation of gibberellic acid derivatives in living cells
Michael J. Ziegler and Richard Wombacher
- Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes
Daniel Z. Wu, Michael A. Lampson and David M. Chenoweth
- Site-specific antibody fragment conjugates for targeted imaging
Robert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang
- Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes
Katherine Charov and Michael D. Burkart
