Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.
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Table of Contents
1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cellsDaniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu
2. Fluorescent labeling of extracellular vesicles
Mehdi Dehghani and Thomas R. Gaborski
3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells
Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai
4. Analysis of individual extracellular vesicles by imaging flow cytometry
Tobias Tertel, Andr? G?rgens and Bernd Giebel
5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy
Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci
6. Methods to purify DNA from extracellular vesicles: Focus on exosomes
Sheila Spada
7. Quantification of mtDNA in extracellular vesicles
Beata Soltes and Balint Nagy
8. Deciphering the total RNA content of extracellular vesicles
Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes
9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids
Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais
10. Isolation and characterization of human amniotic fluid-derived exosomes
Samantha Sheller-Miller and Ramkumar Menon
11. Human urine exosomes: Another important member of the liquid biopsy family
Antonio De Maio
12. Three-dimensional cell models for EV production, isolation, and characterization
Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko
13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles
Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya
14. Live tracking of extracellular vesicles in larval zebrafish
Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz
15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos
Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari
