Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.
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Table of Contents
1. X-ray crystallography shines a light on pore-forming toxins
Michael Parker
2. Giant unilamellar vesicles for studying activity of pore forming proteins
Gregor Anderluh
3. Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphteria Toxin translocation domain
Alexey S. Ladokhin
4. Biophysical approaches to study actinoporin-lipid interactions
Sara Garcia-Linares
5. Molecular basis for activation of Actinoporins by lipids
Kouei Tsumoto
6. Engineered ClyA for detection of biological molecules
Giovanni Maglia
7. Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing
Abdelghani Oukhaled
8. Use of Pore-Forming Toxins to study co-translocational protein folding
David Rodriguez-Larrea
9. The use of pore-forming toxins to image lipids and lipid domains
Toshihide Kobayashi
10. Engineering outer membrane porin for protein detection
Min Chen
11. The use of Ostreolysin A and Anthrolysin O to study cholesterol on cell membranes
Arun Radhakrishnan
12. Studying the mechanism of Pore-forming Toxins using High-speed Atomic Force Microscopy
Simon Scheuring
13. The use of Atomic-Force Microscopy to study Pore-Forming Toxins
Bart Hoogenboom
14. Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membrane
Jialing Lin
15. High-resolution structure and the sample preparation of Clostridium perfringens iota-toxin pore complex
Hideaki Tsuge
16. How protein engineering has revealed the molecular mechanisms of pore-forming toxins?
Rodney Tweten
17. Single Molecule Fluorescence Techniques to Study the Mechanism of Pore-Forming Toxins
Mark Wallace
18. Cryo-EM
Robert Gilbert
19. Going full circle: determining the structures of complement component
Michelle Dunstone
20. Different MD simulation techniques used to study Pore-forming Toxins
Ganapathy Ayappa
21. Contribution of Molecular Dynamics Simulations to the study of Pore Forming Toxins
Gerhard Hummer
22. Membranes with lipid asymmetry for studying Pore-Forming Toxins
Erwin London
23. Topological analysis of Pore-forming proteins in native membranes
Alejandro Heuck
