Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown that bilayers whose leaflets differ in their physical properties-such as composition, phase state, or lateral stress-exhibit many fascinating new characteristics, but also pose a host of new challenges related to their creation, characterization, simulation, and theoretical description. Chapters in this new release include Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS, Effects of lateral and hydrostatic pressure on membrane structure and properties, and much more.
Other sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more.
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Table of Contents
PrefaceTobias Baumgart and Markus Deserno
1. Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS
Nirmalya Bag, Arpita Tripathy and Sudipti Priyadarsinee
2. Fluorescence imaging of lamellipodin mediated biomolecular condensates on solid supported lipid bilayer membranes
Tobias Baumgart
3. Effects of lateral and hydrostatic pressure on membrane structure and properties
Nick Brooks
4. Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells
Itay Budin, Israel Juarez-Contreras and Hyesoo Kim
5. The spectral phasor approach to resolving membrane order with environmentally sensitive dyes
Rumiana Dimova and Agust�n Mangiarotti
6. The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains
Thais A. Enoki
7. Advanced microscopy methods to study membrane pores
Ana J. Garc�a-S�ez
8. Use of cryo-EM to study membrane phase separation
Frederick Heberle and M. Neal Waxham
9. Using lipid binding proteins and advanced microscopy to study lipid domains
TOSHIHIDE KOBAYASHI, NARIO TOMISHIGE, KOHTA TAKAHASHI, BRIGITTE POLLET, LUDOVIC RICHERT and YVES M�LY
10. Structural characterization of lateral phase separation in polymer-lipid hybrid membranes
Cec�lia Leal, Nurila Kambar, Minh N. Do, Corey Snyder and Yoo Kyung Go
11. Applications of phase-separating multi-bilayers in protein-membrane domain interactions
Kandice R. Levental and Hong-Yin Wang
12. Studying lipid flip-flop using NMR and TR-SANS
Drew Marquardt, Dominik Dziura and Maksymilian Dziura
13. Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images
Carlos Marques, Andr� Schroder, Marc Schmutz, Pierre Muller and Eulalie J. Lafarge
14. Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS
Georg Pabst, Enrico Semeraro and Moritz Frewein
15. Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy
Zheng Shi and Shilong Yang
16. A TIRF microscopy-based method for observing the uptake of individual particles by clathrin mediated endocytosis
Jeanne Stachowiak, Grant Ashby, Kayla Keng and Carl Hayden
17. Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity
Claudia Steinem and Larissa Socrier
18. MaCD based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity
Pavana Suresh and Erwin London
