Selenoprotein Structure and Function, Volume 662 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Identification of Selenoprotein O substrates using a biotinylated ATP analog, Selenium-encoded isotopic signature targeted profiling, Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters, Preparation of selenoprotein S by chemical ligation, Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells, SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond, Selenocysteine substitutions in thiyl radical enzymes, and much more.
Additional chapters cover Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain, Metabolic labeling with radioactive selenium in zebrafish, Low pH isoTOP-ABPP to identify selenocysteines, Expression of selenoproteins via genetic code expansion in mammalian cells, Alpha-methyl selenocysteine as a tool for the study of selenoproteins, Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications, and much more.
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Table of Contents
1. Identification of Selenoprotein O substrates using a biotinylated ATP analogAnju Sreelatha
2. Selenium-encoded isotopic signature targeted profiling
Chu Wang
3. Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters
Dieter S?ll
4. Preparation of selenoprotein S by chemical ligation
Sharon Rozovsky
5. Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells
Dohoon Kim
6. SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond
Yaoyang Zhang
7. Selenocysteine substitutions in thiyl radical enzymes
Brandon Greene
8. Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain
Elias S.J. Arn?r
9. Metabolic labeling with radioactive selenium in?zebrafish
Paul Copeland
10. Low pH isoTOP-ABPP to identify selenocysteines
Daniel Bak
11. Expression of selenoproteins via genetic code expansion in mammalian cells
Jennifer Peeler
12. Alpha-methyl selenocysteine as a tool for the study of selenoproteins
Robert Hondal
13. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications
Jianguo Fang and Baoxin Zhang
14. Diselenide-selenoester ligation
Richard Payne
15. Modeling selenocysteine-derived reactive intermediates utilizing a molecular cradle
Kei Goto
16. Methods for accurate and reproducible studies of pharmacological effects of selenium in cancer
Mikael Bj?rnstedt
