Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.
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Table of Contents
1. Production and Purification of the PEAK pseudokinases for structural and functional studies2. Structural biology and biophysical characterisation of Tribbles pseudokinases
3. Detecting endogenous TRIB protein expression and its downstream signalling
4. Analysis of human Tribbles 2 pseudokinase
5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
6. Characterization of pseudokinase ILK-mediated actin assembly
7. Biochemical examination of Titin pseudokinase
8. Approaches to study pseudokinase conformations
9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
10. Proximity ligation approaches to define the interactomes of pseudokinases
11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling
12. Strategies to study and stimulate bacterial pseudokinases
13. Dynamics of Kinases and Pseudokinases by HDX-MS
14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
15. Pseudoenzymology of Kinase Suppressor of Ras
16. Computational and evolutionary insights into pseudokinase mechanisms
17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
18. Pharmacological targeting of the pseudokinase HER3
19. Considerations and analysis of the unusual pseudokinase BUBR1
20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
21. Methods for discovering catalytic activities for pseudokinases
22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
24. Identification of small molecule binders for pseudokinases
25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
