Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
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Table of Contents
1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New TherapiesXiaoyan Jiang
2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR
Guillermo Mazzolini
3. Reverse Phase Protein Array in cancer stem cells
Michele Signore
4. Isolation of cancer stem cells from squamous cell carcinoma
Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC),
5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells
Niccol? Bartalucci
6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity
Paola Marcato
7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs)
Angela Gritti
8. Immunohistochemistry-based analysis of EMT markers in mammary tumors
Diwakar Pattabiraman
9. Immunofluorescence staining of colorectal cancer patient-derived organoids
Joseph L. Regan
10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry
Michael A. Rieger
11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells
Bin Wang Sr., Yingying Lai and Xi Zheng
12. Methods to isolate adipose tissue-derived stem cells
Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco
13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells
Antonella Sistigu
