Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more.
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Table of Contents
1. Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies2. Metabolomic approaches for enzyme function and pathway discovery in bacteria
3. Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry
4. Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance
5. Development and application of highly sensitive labeling reagents for amino acids
6. Bacterial Cell Wall Modification with a Glycolipid Substrate
7. Screening an Established Natural Product Library Identifies Secondary Metabolites that Potentiate Conventional Antibiotics
8. Reactivity-Based Screening for Natural Product Discovery
9. Applications of an Inactive Dispersin B Probe to Monitor Biofilm Polysaccharide Production
10. Synthesis and Application of Fluorescent Teixobactin Analogues
11. Impact of Crossbridge Structure on Peptidoglycan Crosslinking: A Synthetic Stem Peptide Approach
12. Utilizing Imaging Mass Spectrometry to Analyze Microbial Biofilm Chemical Responses to Exogenous Compounds
13. Guidelines for Metabolomics-Guided Transposon Mutagenesis for Microbial Natural Product Discovery
14. Glycopeptide antibiotic discovery in the genomic era
15. Engineered Biosynthesis of Alkyne-tagged Polyketides