Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field. with this new volume presenting chapters on topics including Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases, Near-real time determination of BAHD acyl-coA transferase reaction rates, Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis, Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo, Dirigent Proteins Family Function and Structure, Three-in-One Method for High-throughput plant multi-omics, amongst other timely topics.
Additional sections cover Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry, Lignin/Lignan Biosynthesis Structure/Function, Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species, Plant sphingolipid analysis, RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens, and much more.
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Table of Contents
Protein 1. Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases Michael Sullivan 2. Near-real time determination of BAHD acyl-coA transferase reaction rates Michael Sullivan 3. Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis Michael Sullivan & Amanda Fanelli 4. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo Jeffrey Cameron 5. Dirigent Proteins Family Function and Structure Norman Lewis
Metabolite 6. Three-in-One Method for High-throughput plant multi-omics Sixue Chen 7. Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry Peter Doermann 8. Lignin/Lignan Biosynthesis Structure/Function Norman Lewis 9. Comparsion of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species Phil Bates 10. Plant sphingolipid analysis Jennifer Markam
Environment 11. RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens Shih-Long Tu 12. Monitoring lipid-protein interactions in planta using Forster resonance energy transfer Sam Wang 13. Acetylene reduction assay for nitrogenase activity under salinity stress Senthil Subramanian and Liping Gu 14. Assessing simultaneious reversible cysteine oxidation and global abundance in plant tissues Leslie Hicks 15. High-throughput phenotyping and the use of computer vision to unravel novel phenotypes associated with plant nutrition David Mendoza-Cozatl 16. Using Hyper as a Molecular Probe to visualize hydrogen peroxide in living cells Luis Cardenas 17. A new probe DC_-Rho1 for visualizing protein sulfenylation in living plant cells Luis Cardenas
