Up-to-date, practical reference book demonstrating laboratory procedures in dogs, cats, cattle and horses
The newly revised and updated Second Edition of Clinical Pathology and Laboratory Techniques for Veterinary Technicians is an inclusive reference on laboratory procedures pertaining to small animals, horses, and cattle, providing information on hematology, hemostasis, clinical chemistry, urinalysis, parasitology, endocrine testing, and quality assurance.
The text clarifies ‘how-to’ information while also discussing the importance of ‘why’ laboratory procedures are performed in a particular way, enabling readers to gain true understanding of the topics discussed. To aid in reader comprehension, high-quality photographs labeled with magnification and stain information, which clearly depict cellular morphology, inclusions, and infectious organisms, are included throughout each chapter.
The text also offers key objectives, technician tip boxes, case examples, and a glossary of key terms, along with a companion website that provides instructor questions, and an answer key to multiple choice questions in the book.
Clinical Pathology and Laboratory Techniques for Veterinary Technicians includes information on: - Sample collection, handling, and quality assurance, to ensure laboratory test results are valid- Hematology data collection techniques that may be used on a daily basis to evaluate the overall health of animal patients- Clinical chemistry data to indicate organ function, covering how different chemicals measured in patient samples relate to the function of specific organs in the body- In-house parasitology procedures, with important advances in molecular diagnostics have been made that improve the detection and identification of parasites- Information about common endocrinopathies and submission of samples for endocrine testing.
With complete coverage of the subject, Clinical Pathology and Laboratory Techniques for Veterinary Technicians is an essential resource for students enrolled in veterinary technology programs and veterinary colleges, and may be used as a textbook to guide and supplement information provided in both didactic and laboratory-based clinical pathology courses.
Table of Contents
List of Contributors xi
Preface xiii
About the Companion Website xv
1 Getting Started with Clinical Pathology 1
Amy L. MacNeill
Introduction 2
Standard Equipment 3
Standard Supplies 14
Sample Types 17
Sample Storage and Preparation 17
Basic Laboratory Safety 18
2 Minimizing Laboratory Errors in Veterinary Practice 21
Bente Flatland
Introduction 23
Laboratory Considerations 23
Laboratory Area 26
Types of Laboratory Error 27
Minimizing Laboratory Error 28
Minimizing All Types of Laboratory Error 28
Minimizing Preanalytical Error 31
Minimizing Analytical Error 32
Minimizing Postanalytical Error 38
Summary 39
Further Reading 39
3 Hematology 45
Amy L. MacNeill
Introduction 48
Components of Peripheral Blood 49
Blood Cell Parameters 50
Erythrocytes 51
Leukocytes 68
Platelets 81
Atypical Cells in the Peripheral Blood 84
Blood Parasites 85
Hematology Methods 89
Further Reading 104
4 Hemostasis 109
Amy L. MacNeill
Introduction 112
Physiology 112
Diagnostic Testing 120
Further Reading 129
5 Clinical Chemistry 131
Anne M. Barger
Introduction 132
Available Testing 133
Sample Preparation 134
Sample Quality 137
Clinical Chemistry Health Profiles 140
Purpose 140
Blood Proteins 142
Kidney 147
Liver 153
Muscle 162
Pancreas 163
Glucose 164
Electrolytes 168
Calcium 168
Phosphorus 171
Sodium, Chloride, and Potassium 172
Acid-Base Balance 174
Further Reading 178
6 Urinalysis 181
Anne M. Barger
Introduction 182
Urine Production 183
Concentration of Urine 184
Urine Collection 186
Examination of Urine 188
Urine Concentration 190
Urine Chemistry 193
Urine Sediment 196
Further Reading 217
7 Parasitology 219
Ashley K. McGrew
Introduction 221
Classification of Parasites 222
Life Cycles 223
Effect of the Parasite on the Host 224
Endoparasites 224
Diagnostic Testing 224
Ectoparasites 258
Diagnostic Testing 258
Parasite Identification 263
Further Reading 275
Recommended Websites 276
8 Endocrinology 277
Jérémie Korchia
Introduction 281
Hormonal Physiology 281
Generalities About Hormones 281
Classification of Hormones and Receptors 284
Origin of Endocrine Diseases 285
Samples for Hormone Measurement 288
Submission Form 288
Protocol Considerations 289
Sample Considerations 289
Measurement Methods 290
Endocrine Test Performance and Endocrine Result Interpretation 293
Performance of Immunoassays 293
Difference between Analytical and Clinical Performance 294
Main Endocrine Systems and Associated Diseases/Status in Veterinary Medicine 294
Adrenal System 294
Thyroid System 296
Calcium Regulating System 297
Water Regulatory Systems: Renin-Angiotensin-Aldosterone and ADH Hormone 298
Glucose Metabolism Regulation Systems 300
GH and IGF1 304
Reproductive System 305
Further Reading 311
Glossary of Terms 317
Index 329
