DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist
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Table of Contents
1. Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivationPeter Ly
2. Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents
Stefano Santaguida
3. Genetic reporters to detect and quantify homologous recombination in yeast
Loraine Symington
4. Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants
Genevi�ve Almouzni and Jean-Pierre Quivy
5. Nascent DNA sequencing and its diverse applications in genome integrity research
Andre Nussenzweig and Jacob Paiano
6. Enrichment of DNA replication intermediates by EdU pull down
Ylli Doksani
7. DNA curtains for studying phase separation mechanisms of DNA-organizing proteins
Johannes Stigler
8. Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level
Gheorghe Chistol
9. Cloning and Expansion of Repetitive DNA Sequences
Gideon Coster and Sophie Lyn Williams
10. Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy
Arne Lindqvist
11. An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomes
Stephan Hamperl
12. Monitoring Chk1 kinase activity dynamics in live single cell imaging assays
Olivier Gavet and Vicente Lebrec
13. Visualising DNA damage and repair using single molecule super resolution microscopy
Donna Whelan
14. Quantification of nuclear deformations and DNA damage foci dynamics by live-cell imaging
Francisca Lottersberger
15. Native FISH: A low- and high- throughput assay to analyze the Alternative Lengthening of Telomere (ALT) pathway
Eros Lazzerini-Denchi and Benura Azeroglu
16. Assessment of DNA Fibres
Chris Staples
17. A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions Priyanka Verma 18. Monitoring APOBEC3A protein levels in human cancer cells
John Maciejowski
