Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
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Table of Contents
1. Photoreduction for Rieske oxygenase chemistryPinghua Liu
2. Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen
Thomas B. Hofstetter, Charlotte E. Bopp and Nora M. Bernet
3. Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations
Wei-chen Chang
4. Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy
Alexey Silakov
5. Xe-pressurization studies for revealing substrate-entrance tunnels
Jennifer D. Bridwell-Rabb
6. A tale of two dehydrogenases involved in NADH recycling
Jennifer D. Bridwell-Rabb
7. Rieske oxygenases and/or their partner reductase proteins
Pravindra Kumar
8. Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
Timothy David Bugg
9. Biocatalysis and non-heme iron enzymes
Xiongyi Huang
10. In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01
Sandy Schmidt and Niels A.W. de Kok
11. Structure and function of carbazole 1,9a-dioxygenase
Hideaki Nojiri
12. Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis
Hung-wen Liu
13. Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ
Yisong Guo
14. Non-heme iron oxygenases for natural product biosynthesis
Ikuro Abe
15. Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase
Wenjun Zhang
16. Assays for the epidermal growth factor hydroxylase Asph
Christopher Joseph Schofield
17. Amino acid hydroxylases
Paul Fitzpatrick
18. Extradiol dioxygenases
Yifan Wang
19. Title to be determined
Ramaswamy Subramanian
20. Experimental and computational approaches to understanding and addressing the stability limitations of Rieske Oxygenases
Jennifer DuBois
21. Construction and Testing of Whole-Cell Non-Heme Iron Biocatalysts
Ryan Summers
22. Equilibrium dialysis to measure binding affinities of non-heme iron enzymes
Ambika Bhagi-Damodaran
23. title to be determined
Thomas C. Brunold
24. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase
John D. Lipscomb and Melanie Rogers
25. Preparation of reductases for multicomponent oxygenases
Lindsay David Eltis and Megan Wolf
26. Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases
Shaun McKinnie
27. Title to be determined
Kabirul Islam
28. Title to be determined
Edward I. Solomon
29. title to be detrmined
Adam Offenbacher
