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Mononuclear Non-heme Iron Dependent Enzymes Part B. Methods in Enzymology Volume 704

  • Book

  • September 2024
  • Elsevier Science and Technology
  • ID: 6016387

Mononuclear Non-heme Iron Dependent Enzymes, Volume 704, Part B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Observing extradiol dioxygenases in action through a crystalline lens, Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase, Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, terephthalate dioxygenase, Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions, and much more.

Other sections cover Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes, Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis, Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase, Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis, Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ, and much more.

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Table of Contents

1. Structural studies of mononuclear non-heme iron dependent enzymes
Yifan Wang
2. Observing extradiol dioxygenases in action through a crystalline lens
Ramaswarmy Subramanian
3. Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase
Pravindra Kumar
4. Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, and terephthalate dioxygenase
Adam Offenbacher
5. Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions
Wei-Chen Chang
6. Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes
Hung-wen (Ben) Liu
7. Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis
Wenjun Zhang
8. Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase
Ikuro Abe
9. Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis
Yisong Guo
10. Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ
Shaun McKinnie
11. Expression, purification, and biochemical characterization of micro- and macroalgal kaoinoid synthases
John Lipscomb
12. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase
Timothy Bugg
13. In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
Christopher Schofield
14. Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine--hdyroxylase
Paul Fitzpatrick
15. Biochemical and Biophysical Approaches to Characterization of the Aromatic Amino Acid Hydroxylases