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In Vitro Propagation and Secondary Metabolite Production from Medicinal Plants: Current Trends (Part 2)

  • Book

  • April 2024
  • Bentham Science Publishers Ltd
  • ID: 5960312
This book is a comprehensive review of secondary metabolite production from plant tissue culture. The editors have compiled 12 meticulously organized chapters that provide the relevant theoretical and practical frameworks in this subject using empirical research findings. The goal of the book is to explain the rationale behind in vitro production of secondary metabolites from some important medicinal plants. Biotechnological strategies like metabolic engineering and the biosynthesis, transport and modulation of important secondary metabolites are explained along with research studies on specific plants. In addition to the benefits of secondary metabolites, the book also aims to highlight the commercial value of medicinal plants for pharmaceutical and healthcare ventures.

Topics covered in this part include:

1. In vitro propagation and tissue culture for several plants including Withania somnifera (L.) Dunal, Aloe vera, Oroxylum indicum (L) Kurz, Ocimum basilicum L, Rhubarb, Tea, and many others (including plants in Northern India).

2. Genetic Improvement of Pelargonium

3. Bioactive Components in Senna alata L. Roxb

4. Plant tissue culture techniques

The book caters to a wide readership. It primarily prepares graduate students, researchers, biotechnologists, giving them a grasp of the key methodologies in the secondary metabolite production. It is a secondary reference for support executives, industry professionals, and policymakers at corporate and government levels to understand the importance of plant tissue culture and maximizing its impact in the herbal industry.

Readership

Graduate students and researchers in plant biotechnology courses; industry professionals and policymakers in the herbal industry.

Table of Contents

CHAPTER 1 BIOACTIVE COMPONENTS IN SENNA ALATA L. ROXB
  • Archana Pamulaparthi, Vamshi Ramana Prathap and Ramaswamy Nanna
  • INTRODUCTION
  • Results and Discussion
  • Physicochemical Parameters
  • Phytochemical Analysis
  • DISCUSSION
  • CONCLUSION
  • REFERENCES
CHAPTER 2 PLANT TISSUE CULTURE: A POTENTIAL TOOL FOR THE PRODUCTION
  • OF SECONDARY METABOLITES
  • Madhukar Garg, Soumi Datta and Sayeed Ahmad
  • INTRODUCTION
  • SECONDARY METABOLITES
  • Primary vs Secondary Metabolites
  • The Biosynthesis of Secondary Metabolites
  • The Physiological Function of Secondary Metabolites
  • Classification of Secondary Metabolites
  • Alkaloids
  • Terpenoids
  • Steroids
  • Quinones
  • Phenylpropanoid
  • Secondary Metabolites and Plant Tissue Culture
  • Callus Cultures
  • Organ Cultures
  • Cell Suspension Cultures
  • Fungal Elicitation
  • Micropropagation
  • Strategies to Increase in vitro Synthesis of Secondary Metabolite
  • Conventional Strategies
  • Biotechnological Strategies (Metabolic Engineering, etc.)
  • Use of Nanoparticles for Secondary Metabolite (s) Enhancement
  • Large-scale Production of SMs by Bioreactors
  • CONCLUSION AND PERSPECTIVES
  • ABBREVIATIONS
  • REFERENCES
CHAPTER 3 IN VITRO PROPAGATION AND SECONDARY METABOLITE
  • PRODUCTION FROM WITHANIA SOMNIFERA (L.) DUNAL
  • Praveen Nagella, Wudali Narashima Sudheer and Akshatha Banadka
  • INTRODUCTION
  • BIOSYNTHESIS OF WITHANOLIDES
  • IN VITRO PROPAGATION STUDIES
  • Direct Organogenesis
  • Indirect Organogenesis
  • Rooting of the Regenerated Plantlets
  • Acclimatization
  • IN VITRO PRODUCTION OF WITHANOLIDES
  • Production of Withanolides from Cell/Callus Culture
  • Production of Withanolides from Shoot Culture
  • Production of Withanolides from Adventitious Root Culture
  • Production of Withanolides from Hairy Root Culture
  • ELICITATION STRATEGIES FOR IMPROVED PRODUCTION OF WITHANOLIDES 79
  • METABOLIC ENGINEERING FOR INCREASED PRODUCTION OF WITHANOLIDES 81
  • GENETIC TRANSFORMATION STUDIES IN WITHANIA SOMNIFERA
  • CONCLUSION AND PROSPECTS
  • REFERENCES
CHAPTER 4 IN VITRO PROPAGATION AND PHYTOCHEMICAL SCREENING OF SOME
  • IMPORTANT MEDICINAL PLANTS OF NORTHERN INDIA-A REVIEW
  • Rafiq Lone, Shakir Ahmad Mochi, Younis Ahmad Hajam, Ibraq Khurshid and Azra
  • N. Kamili
  • INTRODUCTION
  • Acorus calamus L
  • Alcea rosea L. (Malvaceae)
  • Hyoscyamus niger L.
  • Gymnema sylvestre R. Br.
  • Abies pindrow (Royle ex D.Don)
  • Heracleum candicans Wall.
  • Jurinea dolomiaea Boiss
  • Arnebia benthamii Wall. ex G. Don
  • Atropa acuminata Royle Ex Lindl.
  • Meconopsis aculeta Royle L.
  • SUMMARY AND CONCLUSION
  • REFERENCES
CHAPTER 5 PHYTOCHEMISTRY, ANTIOXIDANTS, ANTIMICROBIAL ACTIVITIES
  • AND EDIBLE COATING APPLICATION OF ALOE VERA
  • Awad Y. Shala, Hayam M. Elmenofy, Eman Abd El-Hakim Eisa and Jameel M. Al-
  • Khayri
  • INTRODUCTION
  • BIOACTIVE COMPONENTS
  • FACTORS AFFECTING PLANT CHEMICAL COMPOSITION
  • ANTIOXIDANT ACTIVITY OF A. VERA
  • ANTIMICROBIAL ACTIVITY
  • Antibacterial Activity
  • Antifungal Activity
  • ALOE VERA AS AN EDIBLE COATING FOR VEGETABLES AND FRUITS
  • Effect of A. vera Coating on Microbial Decay and Physiological Disorders of Fruits
  • Microbial Activity
  • Physiological Disorders
  • Impact of Aloe vera Coatings on Fruit and Vegetables' Physical-chemical Qualities
  • Fruit Firmness
  • Soluble Solids Content and Acidity
  • Respiration and Ethylene Production
  • Sensory Attributes and Color
  • Total Phenolic Content and Antioxidant Activity
  • IN VITRO PROPAGATION RESPONSES OF ALOE VERA
  • Microstructural and Histochemical Changes in Aloe vera from In Vitro to In Vivo
  • CONCLUSION
  • ACKNOWLEDGMENTS
  • REFERENCES
CHAPTER 6 MICROPROPAGATION AND PHYTOCHEMICAL STUDIES ON OROXYLUM
  • INDICUM (L) KURZ - A REVIEW
  • Samatha Talari and Rama Swamy Nanna
  • INTRODUCTION
  • In-vitro Seed Germination
  • Zygotic Embryo Culture
  • Clonal Propagation
  • Nodal Culture
  • Mericlone Technology
  • In vitro Rooting and Plantlet Establishment
  • Callus Induction
  • Organogenesis
  • Somatic Embryogenesis
  • Phytochemical Studies
  • Toxicological Activity
  • Antimicrobial Activity
  • Antioxidant Activity
  • Anti-inflammatory Activity
  • Hepatoprotective Activity
  • Nephroprotective Activity
  • Antidiabetic Activity
  • Immunomodulatory Activity
  • Anticancer Activity
  • Anti-angiogenic Activity
  • CONCLUSION
  • LIST OF ABBREVIATIONS
  • REFERENCES
CHAPTER 7 EXPLORING PLANT TISSUE CULTURE IN OCIMUM BASILICUM L.
  • Priyanka Chaudhary, Shivika Sharma and Vikas Sharma
  • INTRODUCTION
  • EVALUATION OF HEREDITARY STABILITY OF TISSUE CULTURE RAISED
  • PLANTLETS VIA MOLECULAR MARKERS
  • SHOOT INDUCTION
  • In Vitro Rooting
  • ACCLIMATIZATION
  • SOMATIC EMBRYOGENESIS
  • PRODUCTION OF SECONDARY METABOLITES IN O.BASILICUM
  • ORGAN AND CALLUS CULTURE
  • CELL SUSPENSION CULTURE
  • IN VITRO PLANT CELL ELICITATION
  • CONCLUSION AND FUTURE PERSPECTIVES
  • REFERENCES
CHAPTER 8 PLANT TISSUE CULTURE: A PERPETUAL SOURCE FOR THE
  • PRODUCTION OF THERAPEUTIC COMPOUNDS FROM RHUBARB
  • Shahzad A. Pandith and Mohd. Ishfaq Khan
  • INTRODUCTION
  • RHUBARB: A General Account
  • ETHNOBOTANY AND PHARMACOLOGY OF RHUBARB
  • PHYTOCONSTITUENTS FROM RHUBARB WITH THERAPEUTIC SIGNIFICANCE 207
  • Flavonoids
  • Anthraquinones
  • Stilbenoids
  • IN VITRO PROPAGATION OF RHUBARB: CHALLENGES AND PROSPECTS
  • SOMATIC EMBRYOGENESIS AS AN ALTERNATIVE TO IN VITRO
  • MICROPROPAGATION OF RHUBARB
  • INITIATION AND ESTABLISHMENT OF CELL SUSPENSION CULTURES: A
  • PROSPECTIVE APPROACH FOR RHUBARB
  • IN VITRO SYSTEMS FOR THE PRODUCTION OF SECONDARY METABOLITES IN
  • RHUBARB
  • PRECURSOR ADDITION AS A MEANS TO IMPROVE THE PRODUCTION OF BIO-
  • ACTIVE CONSTITUENTS IN RHUBARB
  • HAIRY ROOTS: A POTENTIAL SOURCE FOR SECONDARY CHEMICAL
  • CONSTITUENTS
  • CONCLUSION AND FUTURE PROSPECTS
  • REFERENCES
CHAPTER 9 IN VITRO PLANT REGENERATION FROM NODAL SEGMENTS AND
  • BIOCHEMICAL FIDELITY ANALYSIS OF OPERCULINA TURPETHUM, A THREATENED
  • MEDICINAL PLANT OF ODISHA
  • Kumari Monalisa, Shashikanta Behera, Shasmita, Debasish Moha-patra, Anil K.
  • Biswal and Soumendra K. Naik
  • INTRODUCTION
  • MATERIALS AND METHODS
  • Collection and Surface Sterilization of the Explants
  • Culture Medium and Culture Conditions
  • Acclimatization of Plantlets of O. turpethum
  • Phytochemical Analysis
  • Preparation of the Sample
  • Data Recording and Statistical Analysis
  • RESULTS
  • Evaluation of Growth Regulators for Axillary Shoot Proliferation
  • Rooting of in vitro Regenerated Shoots
  • Acclimatization of in vitro Regenerated Plantlets
  • Phytochemical Analysis
  • DISCUSSION
  • CONCLUSION
  • ACKNOWLEDGEMENT
  • REFERENCES
CHAPTER 10 TISSUE AND CELL CULTURE OF TEA (CAMELLIA SP.)
  • Abhishek Mazumder, Urvashi Lama, Meghali Borkotoky, Sangeeta Borchetia, Shabana
  • Begam and Tapan Kumar Mondal
  • INTRODUCTION
  • MICROPROPAGATION OF TEA
  • Stage I: Selection of Explants and Establishment of Culture Through Explants
  • Stage II: Initiation, Multiplication, and Elongation of Shoots

Author

  • Mohammad Anis
  • Mehrun Nisha Khanam