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Harnessing Genomic Tools for Crop Improvement. A Software User Manual for Transforming Geneticists to Genomicists

  • Book

  • August 2025
  • Elsevier Science and Technology
  • ID: 6051699
Harnessing Genomic Tools for Crop Improvement: A Software User Manual for Transforming Geneticists to Genomicists is an essential resource aimed at users of crop improvement software. This manual offers comprehensive guidance to understand and utilize molecular data derived from genetics and breeding experiments. By presenting step-by-step instructions for various genomics-related software, such as genetic diversity (DARWIN) and QTL mapping (QTL Cartographer), it serves both novice and expert users. The book simplifies the complicated processes of genome-wide association mapping, haplotype analysis, and marker-assisted breeding, ensuring accurate interpretation and application of the results.

In addition to the step-by-step guides, the book explores cutting-edge bioinformatics tools such as databases, BLAST, primer designing, and sequence alignment. Definitions and terminology clarifications are provided, along with detailed interpretations of the software outputs through case studies. This comprehensive manual is invaluable to scientists, students, and post-doctoral fellows interested in plant and animal genetics, making it a vital asset in the field of genomics.

Table of Contents

1. Basic bioinformatics software
2. Molecular marker development
3. ARMS-PCR, CAPS, dCAPS, etc 3. NGS analysis
4. Genetic diversity and population structure analysis
5. QTL mapping
6. Genome-wide association mapping
7. Statistical models for Genomic selection
8. Haplotype analysis
9. Analysis of Gene regulatory networks
10. Transcriptome analysis
11. MiRNA structure and target prediction tools
12. Designing of guide RNA for CRISPR gene editing
13. Protein Interaction Network Analysis: Essential Tools and Resources
14. Navigating in silico drug discovery: A hands-on guide with Auto Dock Vina, ChemDraw and Chimera for structure simulation
15. Machine learning techniques for gene prediction

Authors

Lakshmi Narayana R. Vemireddy S.V. Agricultural College, India.

Dr. Lakshmi Narayana R. Vemireddy obtained an M.Sc. (Ag) from Genetics and Plant Breeding, S.V. Agricultural College, ANGRAU, Tirupati in 2000 and a Ph.D. from the Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad (Registered in University of Hyderabad) in 2008 and Post-Doctoral Fellow in Oklahoma State University, Stillwater, USA in 2014. He has more than two decades of proven research experience in rice molecular genetics genomics, and breeding. He is also well acquainted with advanced molecular biology techniques as evident from his publications in reputed international journals. He did his Post-Doctoral Fellowship at Oklahoma State University, Stillwater, OK, USA under "Raman Post-Doctoral Fellowship� sponsored by the University Grants Commission (UGC), New Delhi. In addition, he also underwent three months of international training under the Institutional Development Plan of ANGRAU - NAHEP at the University of Queensland, Brisbane, Australia.

Bommisetty Reddyyamini S.V. Agricultural College, India.

Dr. Bommisetty Reddyyamini completed her postgraduate degree from the Agricultural College, Mahanandi, and her Doctoral degree from S.V. Agricultural College, Tirupati, ANGRAU, Guntur in 2012 and 2019, respectively. She was actively involved in conducting molecular plant breeding practical experiments and handles bioinformatics courses for postgraduate and Ph.D. students. She received the "Outstanding Ph.D. thesis award� at the second international conference on Advancement of Science and Technology for Environment, Society and People (ICASTESP-2023) held in 2023.

Yellari Amaravathi S.V. Agricultural College, India.

Dr. Yellari Amaravathi has research experience in molecular breeding, tissue culture, genetic engineering, and genomics aspects. She obtained her M.Sc. (Ag) from Plant Genetic Resource, National Bureau of Plant Genetic Resources (NBPGR), IARI, New Delhi in 2001. She pursued her Doctoral program at the National Institute of Plant Biotechnology (formerly NRCPB), IARI, New Delhi in 2006. She started her career by joining the Defence Agricultural Research Laboratory (DARL), Ministry of Defence as Scientist 'B' in 2005 and was involved in genetic engineering of cold tolerance in vegetable crops like Tomatoes, Bell peppers, cucumbers, and radishes. She joined ANGRAU as Assistant Professor in 2013 and was involved in both teaching and research. Her research interests are in understanding molecular mechanisms in plant-pathogen interactions, exploring genes to develop durable resistance, and exploitation of novel technologies such as CRISPR/Cas9 for crop improvement.