Results for tag: "Gentle Cell Dissociation Reagent"

In the field of biotechnology, Gentle Cell Dissociation Reagent is utilized for the detachment of adherent cells from the substrate or surface to which they are attached. This process is crucial for various applications, including cell culture, research, and therapeutic uses. Gentle dissociation reagents are designed to preserve cell membranes and surface proteins, ensuring high viability and functionality of cells post-dissociation, which is particularly important in sensitive downstream applications such as flow cytometry, cell sorting, and cell therapies. These reagents are composed of enzymes or non-enzymatic solutions that facilitate cell detachment by disrupting protein-protein and protein-surface interactions with minimal impact on cell health. Enzymatic reagents, such as trypsin, are commonly used; they cleave peptide bonds in the proteins mediating cell adhesion. Non-enzymatic reagents are typically chelating agents that remove divalent cations essential for cell adhesion, thus allowing for cell detachment without enzymatic activity, which can be preferable for preserving certain cell surface markers. Some leading companies in the Gentle Cell Dissociation Reagent market include Thermo Fisher Scientific, which offers a range of products under the Gibco brand, BioLegend with its gentle dissociation solutions tailored for specific cell types, Roche with its Accutase cell dissociation reagents, and STEMCELL Technologies, known for their enzyme-free dissociation solutions. Together, these and other companies support the evolving needs of researchers and clinicians in the Show Less Read more