Modifications and Targeting of Protein Termini Part A. Methods in Enzymology Volume 684

Table of Contents

1. Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells Bernd Bukau, Jiri Koubek, Manuel G?nnigmann and G?nter Kramer 2. Deformylation of nascent chains on the ribosome Marina V. Rodnina, Lena B?geholz, Evan Mercier and Wolfgang Wintermeyer 3. Optimizing purification and activity assays of N-terminal methyltransferase complexes Christine Schaner Tooley, Haley Parker and John Tooley 4. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods Rong Huang and Ying Meng 5. Biochemical and structural analysis of N-myristoyltransferase-mediated protein tagging Thierry Meinnel, Carmela Giglione, Paul Monassa, Fr?d?ric Rivi?re, Cyril Dian and Fr?d?ric Frottin 6. Kinetic and catalytic features of N-myristoyltransferases Thierry Meinnel and Carmela Giglione 7. Detecting N-terminal myristoylation using metabolic Alk12 labeling Hening Lin and Yilai Xu 8. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities Iris Finkemeier, J?rgen Eirich, Julia Sindlinger, Stefan Sch?n and Dirk Schwarzer 9. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms Kris Gevaert, Annelies Bogaert, Tessa Van de Steene, Marnik Vuylsteke and Sven Eyckerman 10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification, interaction studies and enzymatic assays Ruth Geiss-Friedlander, Laura Donzelli and Oguz Bolgi